Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Highly active, lipase silicone elastomers.

Amro M Ragheb1, Michael A Brook, Michael Hrynyk

  • 1Department of Chemistry, McMaster University, 1280 Main St. W., Hamilton, Ont., Canada L8S 4M1.

Biomaterials
|December 4, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tuning the viscoelasticity of sugar-silicone polymers using caramelization.

Chemical communications (Cambridge, England)·2026
Same author

Strategies to Improve the Sustainability of Silicone Polymers.

Macromolecules·2025
Same author

Formulation development of a stable influenza recombinant neuraminidase vaccine candidate.

Human vaccines & immunotherapeutics·2024
Same author

Functional silicone oils and elastomers: new routes lead to new properties.

Chemical communications (Cambridge, England)·2023
Same author

Exploring Lipoic Acid-Mediated Dynamic Bottlebrush Elastomers as a New Platform for the Design of High-Performance Thermally Conductive Materials.

ACS applied materials & interfaces·2023
Same author

Antioxidant Silicone Elastomers without Covalent Cross-Links.

ACS sustainable chemistry & engineering·2023
Same journal

Remodeling TME via feedback-driven photothermal-ferroptosis-immune cascade.

Biomaterials·2026
Same journal

Corrigendum to "Photodynamic therapy produces enhanced efficacy of antitumor immunotherapy by simultaneously inducing intratumoral release of sorafenib" [Biomaterials 2020, 240, 119845].

Biomaterials·2026
Same journal

Mg-integrated octopus-inspired hydrogel dressing enables autonomous adhesion and wound closure for enhanced healing via sequential microenvironment regulation.

Biomaterials·2026
Same journal

Engineering miRNA-223 nanocomplexes via bioorthogonal self-assembly for precision therapy of intervertebral disc degeneration.

Biomaterials·2026
Same journal

Corrigendum to "Enhanced fluorescence imaging guided photodynamic therapy of sinoporphyrin sodium loaded graphene oxide" [Biomaterials 42 (2015) 16442].

Biomaterials·2026
Same journal

An injectable Ce-MnCo LDH nanozyme gel with cascade catalytic activity for acute radiation proctitis in rats.

Biomaterials·2026
See all related articles

This study shows that hydrophobic silicones protect and activate lipase (Candida rugosa), enhancing its activity in silicone elastomers. Hydrophilic additives decrease enzyme dispersion and activity, but silicone oil pre-treatment mitigates this effect.

Area of Science:

  • Biocatalysis
  • Polymer Science
  • Enzyme Immobilization

Background:

  • Enzyme immobilization is crucial for industrial biocatalysis.
  • Silicone elastomers offer unique hydrophobic environments for enzyme stabilization.
  • Understanding the impact of material properties on immobilized enzyme activity is essential.

Purpose of the Study:

  • To investigate the immobilization of Candida rugosa lipase in silicone rubber.
  • To evaluate the influence of various factors (hydrophilicity, crosslinker/enzyme concentration, thickness) on enzyme activity.
  • To determine the role of hydrophobic silicones in enzyme protection and activation.

Main Methods:

  • Lipase Candida rugosa was entrapped in silicone rubber using room temperature vulcanization.

Related Experiment Videos

  • The effects of water, poly(ethylene oxide), crosslinker concentration, enzyme concentration, and elastomer thickness were systematically studied.
  • Enzyme activity was measured in silicone elastomers, silicone oils, and hydrocarbon solvents.

    • Main Results:

    • Lipase exhibited higher activity in silicone elastomers and oils compared to hydrocarbons.
    • Crosslink density did not significantly impact enzyme reactivity.
    • Incorporating hydrophilic moieties reduced enzyme dispersion and activity, an effect lessened by pre-treating lipase with silicone oil.

    Conclusions:

    • Hydrophobic silicones provide a protective and activating environment for lipase.
    • The presence of hydrophilic additives negatively affects enzyme immobilization and activity in silicone matrices.
    • Silicone oil pre-treatment can enhance lipase compatibility with hydrophobic silicone elastomers.