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Related Experiment Videos

The effect of non-specific interactions on cellular adhesion using model surfaces.

Mark H Lee1, David A Brass, Ronit Morris

  • 1Department of Bioengineering, University of Pennsylvania, 120 Hayden Hall, 3320 Smith Walk, Philadelphia, PA 19104, USA. markhl@seas.upenn.edu

Biomaterials
|December 4, 2004
PubMed
Summary

Non-specific cell adhesion is strongly influenced by surface chemistry. Amine-terminated self-assembled monolayers (SAMs) showed significantly higher cell adhesion than other functional groups, highlighting substrate importance in cell studies.

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Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cell Biology

Background:

  • Understanding non-specific cell adhesion is crucial for biomaterial development and cell-based assays.
  • Model functional surfaces are essential tools for dissecting cellular interactions.
  • Self-assembled monolayers (SAMs) offer tunable surface properties for controlled experiments.

Purpose of the Study:

  • To quantify the contribution of non-specific interactions between K100 erythroleukemia cells and model functional surfaces.
  • To investigate the influence of different surface chemistries on cell adhesion strength.
  • To compare non-specific adhesion to peptide-mediated adhesion.

Main Methods:

  • Fabrication of self-assembled monolayers (SAMs) with epoxide, carboxyl (COOH), amine (NH(2)), and methyl (CH(3)) functional groups.

Related Experiment Videos

  • Characterization of SAMs using ellipsometry, atomic force microscopy, contact angle goniometry, and X-ray photoelectron spectroscopy.
  • Measurement of cell adhesion strength using a spinning disc apparatus.

    • Main Results:

    • SAMs exhibited expected physicochemical characteristics and stability in aqueous conditions.
    • Cell adhesion strength followed the trend: CH(3) < COOH ≈ epoxide << NH(2).
    • Amine (NH(2)) terminated SAMs demonstrated an order of magnitude greater non-specific cell adhesion compared to other surfaces, exceeding RGD peptide-mediated adhesion.

    Conclusions:

    • Surface chemistry significantly impacts non-specific cell adhesion.
    • Amine functional groups promote strong non-specific cell adhesion.
    • Careful substrate selection is critical for accurate quantitative studies of cell adhesion, particularly in peptide-mediated interactions.