Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Apple procyanidins decrease cholesterol esterification and lipoprotein secretion in Caco-2/TC7 enterocytes.

Romain Vidal1, Sandra Hernandez-Vallejo, Thomas Pauquai

  • 1Unité Mixte de Recherche 505, Institut National de la Santé et de la Recherche Médicale-Université Pierre et Marie Curie/Ecole Pratique des Hautes Etudes, 75006 Paris, France.

Journal of Lipid Research
|December 4, 2004
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risk factors for acquisition of SARS-CoV-2 Omicron variant among a prospective cohort of French Healthcare Workers: impact of hybrid immunity.

Scientific reports·2026
Same author

[Liquid biopsy: Time to scale up academic education].

Bulletin du cancer·2026
Same author

SOD mimics delivered to the gut using lactic acid bacteria mitigate the colitis symptoms in a mouse model of inflammatory bowel diseases.

Free radical research·2025
Same author

Kinetic Redox Shotgun Proteomics Reveals Specific Lipopolysaccharide Effects on Intestinal Epithelial Cells, Mitigated by a Mn Superoxide Dismutase Mimic.

Angewandte Chemie (International ed. in English)·2025
Same author

Lipids, from molecular mechanisms to diseases.

Biochimie·2024
Same author

Analytical interference in CA 19-9 immunoassay generates false-positive results in a young woman with a low-grade appendiceal tumor: An educational case report and brief literature review.

Annales de biologie clinique·2024

Apple polyphenols, but not wine, reduce intestinal lipoprotein secretion by inhibiting cholesterol esterification and apolipoprotein B synthesis. This suggests a mechanism for their lipid-lowering effects in vivo.

Area of Science:

  • Nutritional Science
  • Molecular Biology
  • Gastroenterology

Background:

  • Polyphenols are known to lower plasma lipids, potentially via effects on hepatic lipoprotein secretion.
  • The intestine plays a crucial role in lipid homeostasis through postprandial secretion of triglyceride-rich lipoproteins.
  • The impact of dietary polyphenols on intestinal lipoprotein metabolism remains less understood.

Purpose of the Study:

  • To investigate the effects of apple and wine polyphenol extracts on lipoprotein synthesis and secretion in human intestinal cells.
  • To elucidate the specific mechanisms by which polyphenols influence intestinal lipid handling.

Main Methods:

  • Utilized human Caco-2/TC7 enterocytes cultured in vitro.
  • Apically supplied cells with complex lipid micelles to mimic postprandial conditions.

Related Experiment Videos

  • Quantified cholesterol esterification, apolipoprotein B (apoB) synthesis and secretion, and related enzyme activities.

    • Main Results:

    • Apple polyphenol extract dose-dependently inhibited cholesterol esterification and lipoprotein secretion in enterocytes.
    • Apple polyphenols reduced apolipoprotein B (apoB) secretion by inhibiting its synthesis, without increasing degradation.
    • Neither cholesterol uptake nor apoB mRNA levels were affected; however, apple procyanidins mimicked the inhibitory effects.

    Conclusions:

    • Apple polyphenols, particularly procyanidins, impair intestinal lipoprotein secretion by inhibiting cholesterol esterification and apoB synthesis.
    • This mechanism suggests impaired lipid availability contributes to the hypolipidemic effects of apple polyphenols observed in vivo.
    • Wine polyphenols did not exhibit similar inhibitory effects on intestinal lipoprotein metabolism under these experimental conditions.