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Isolation of angiotensin converting enzyme (ACE) inhibitory flavonoids from Sedum sarmentosum
Hyuncheol Oh1, Dae-Gill Kang, Ji-Woong Kwon
1Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.
Abstract:
Bioassay-guided fractionation of the EtOAc-soluble extract of Sedum sarmentosum afforded a new flavonoid, quercetin-3-O-alpha-(6'''-caffeoylglucosyl-beta-1,2-rhamnoside) (1), along with four known flavonoids, quercetin 3-O-alpha-(6'''-p-coumaroylglucosyl-beta-1,2-rhamnoside) (2), isorhamnetin-3-beta-glucopyranoside (3), quercetin-3-beta-glucopyranoside (4), and kaempferol-3-alpha-arabinopyranoside (5). Purification of these compounds was conducted with the application of various chromatographic methods. Compounds 1-5 inhibited angiotensin I converting enzyme (ACE) activity in a concentration-dependent manner. Compounds 1-5 had 50% inhibitory concentration values of 158.9+/-11.1 microgM, 351.6+/-3.9 microgM, 408.9+/-4.6 microgM, 708.8+/-23.1 microgM, and 392.8+/-13.4 microgM.
