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Updated: Aug 19, 2026

A Reversible, Non-invasive Method for Airway Resistance Measurements and Bronchoalveolar Lavage Fluid Sampling in Mice
Published on: April 13, 2010
[Cold provocation test. A method for diagnosis of bronchial asthma]
E Bach-Gansmo1, O Skjønsberg, N P Boye
1Avdeling for lungesykdommer og allergi, Oslo.
Abstract:
This article describes the method of isocapnic hyperventilation, where cold air (-22 degrees C) containing 5% CO2 is used to induce a temporary obstruction of airways in patients with bronchial hyperreactivity. The method is quick, has few side effects and is suitable both in the diagnosis and assessment of asthma. Tested out on ten current asthmatics and 14 controls, a median fall in forced expiratory volume (FEV1) of 21.7% was observed in the asthmatic group as against a median fall in FEV1 of 1.4% among the controls.
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