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A simple technique for postembedding ultrastructural immunogold labeling
1Department of Pathology, University of Mississippi Medical Center, Jackson 39216.
Ultrastructural Pathology
|January 1, 1992
Abstract:
A simple and reproducible method for postembedding ultrastructural immunogold labeling is described. Tissues are fixed in Carson-Millonig phosphate-buffered 4% formaldehyde, and then are embedded in LR White resin. Immunogold labeling of ultrathin sections is accomplished by means of an indirect method that employs a nonconjugated primary antibody and gold particles conjugated to antibody to immunoglobulin G. Technical aspects of fixation, embedding, and immunolabeling that influence the success of this protocol are discussed.