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A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
Using mass spectrometry to probe the subtle differences in conformations of several cytochromes c in aqueous and
Yao-Fong Wang1, May-Yeh Ho, Yen-Peng Ho
1Department of Chemistry, National Dong Hwa University, Hualien, Taiwan.
Abstract:
We have detected, using electrospray mass spectrometry, minor changes in the H/D exchange rates in various solvents for cytochromes c obtained from five different species. We compared the exchange rates exhibited by these proteins by mixing horse cytochrome c with each of the other four species and monitoring their exchanges simultaneously by mass spectrometry. The use of horse cytochrome c as a reference allowed us to make very accurate comparisons of the small differences in hydrogen exchange rates among the various species. The exchange experiments were performed in water and methanol at several concentrations in an effort to determine whether the cytochromes c of these five species have different conformations in specific solvents, which would cause their exchange rates to differ. Therefore, monitoring the level of exchange as a function of time in both water and water-methanol mixtures is a method for detecting subtle structural changes of proteins in their native or unfolded intermediate states.
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