Related Experiment Video

Updated: Aug 20, 2026

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Akt and 14-3-3eta regulate Miz1 to control cell-cycle arrest after DNA damage

Michael Wanzel1, Daniela Kleine-Kohlbrecher, Steffi Herold

  • 1Institute for Molecular Biology and Tumor Research, University of Marburg, Emil-Mannkopff-Strasse 2, 35033 Marburg, Germany.

Nature Cell Biology
|December 8, 2004
PubMed

Insights

The transcription factor Miz1 halts cell-cycle progression after DNA damage. A newly identified gene, 14-3-3eta, inhibits Miz1, impacting cell recovery from DNA damage arrest.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • The transcription factor Miz1 plays a crucial role in DNA-damage-induced cell-cycle arrest.
  • Understanding the regulation of Miz1 is vital for comprehending cellular responses to DNA damage.

Purpose of the Study:

  • To identify novel regulators of Miz1 function in response to DNA damage.
  • To elucidate the mechanism by which Miz1's function is modulated, affecting cell-cycle arrest and recovery.

Main Methods:

  • Identification of interacting partners of Miz1.
  • Analysis of gene expression changes upon DNA damage.
  • Investigation of protein-protein interactions and phosphorylation events (Akt).

Main Results:

  • 14-3-3eta was identified as a novel inhibitor of Miz1.
  • 14-3-3eta binds to Miz1's DNA binding domain, dependent on Akt phosphorylation.
  • Miz1 exhibits dual roles: c-Myc-regulated gene upregulation and Akt/14-3-3eta-regulated gene repression.

Conclusions:

  • 14-3-3eta negatively regulates Miz1's role in repressing gene expression during DNA damage response.
  • Akt-mediated phosphorylation of Miz1 is critical for 14-3-3eta binding and modulation of gene repression.
  • This interaction impacts the cell's ability to recover from DNA damage-induced arrest.

More Related Videos

Visualization of DNA Repair Proteins Interaction by Immunofluorescence07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...