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Probing ligand-protein recognition with sum-frequency generation spectroscopy: the avidin-biocytin case

Laurent Dreesen1, Yannick Sartenaer, Christophe Humbert

  • 1Laboratoire de Spectroscopie Moléculaire de Surface, University of Namur, rue de Bruxelles, 61, 5000 Namur, Belgium. laurent.dreesen@fundp.ac.be

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|December 8, 2004
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...