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Related Experiment Videos

Development of sludge based stable aqueous Bacillus thuringiensis formulations.

S K Brar1, M Verma, R D Tyagi

  • 1INRS-ETE, Université Du Québec 2700 rue Einstein, Sainte-Foy Québec, G1V 4C7, Canada.

Water Science and Technology : a Journal of the International Association on Water Pollution Research
|December 8, 2004
PubMed
Summary

Developing stable Bacillus thuringiensis (Bt) biopesticides from wastewater sludge is crucial for sustainable agriculture. This study optimized aqueous flowable formulations, identifying key components for enhanced stability and efficacy.

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The Poly(3-Hydroxybutyrate) Production From the Mixed Culture With the Leachate Obtained From Wastewater Sludge Pretreated by the Electrochemical Process.

Biotechnology and applied biochemistry·2025

Area of Science:

  • Agricultural Science
  • Environmental Microbiology
  • Biotechnology

Background:

  • Wastewater sludge presents a potential source for developing cost-effective biopesticides.
  • Bacillus thuringiensis (Bt) is a widely used microbial agent for insect control.
  • Developing stable, flowable formulations is essential for practical application of biopesticides.

Purpose of the Study:

  • To develop stable aqueous flowable formulations for Bacillus thuringiensis (Bt) biopesticides using wastewater sludge.
  • To evaluate the impact of various inert ingredients on formulation stability and efficacy.
  • To determine optimal storage conditions (pH and temperature) for the developed biopesticide formulations.

Main Methods:

  • Screening of inert ingredients including sorbitol, sodium monophosphate, and Tween-80.

Related Experiment Videos

  • Preparation and testing of five different non-hydrolyzed secondary sludge formulations.
  • Evaluation of physical parameters (viscosity, particle size, suspendibility), entomotoxicity, and microbiological purity.
  • Stability testing of formulations under various pH (4.0-6.5) and temperature (4-50°C) conditions over 120 days.

    • Main Results:

    • The F5 formulation, containing sorbitol, sodium monophosphate, and sodium metabisulphite, exhibited superior physical and biological characteristics.
    • Formulations showed better stability at pH 4.0-5.0 and temperatures of 4-30°C.
    • Degeneration of the product was observed at pH 6.0-6.5 and temperatures of 40-50°C, attributed to proteolytic activity and changes in ionic strength.

    Conclusions:

    • Optimized formulations using specific inert ingredients can enhance the stability and shelf-life of Bt biopesticides derived from wastewater sludge.
    • Storage conditions significantly impact the stability of these biopesticide formulations, with acidic pH and moderate temperatures being optimal.
    • Further research into mitigating degradation factors at higher pH and temperatures is warranted for broader application.