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Updated: Aug 7, 2026

A Facile Protocol to Generate Site-Specifically Acetylated Proteins in Escherichia Coli
Published on: December 9, 2017
Unchanged acetylation of isoniazid by alcohol intake
J T R Wilcke1, M Døssing, H R Angelo
1Internal Medicine I, Bispebjerg Hospital, Copenhagen, Denmark. t.wilcke@inet.uni2.dk
Setting:
In 10 healthy subjects, the influence of acute alcohol intake on the pharmacokinetics of isoniazid (INH) was studied.
Objective:
To test the hypothesis that alcohol increases the conversion of INH by acetylation into its metabolite acetylisoniazid.
Design:
In a crossover design, an oral dose of 300 mg INH was administered on 2 separate days, 14 days apart, with or without alcohol to a serum alcohol of about 21 mmol/l (1 g/l) maintained for 12 h.
Results:
Neither the metabolism of INH nor that of acetylisoniazid was changed by acute alcohol intake.
Conclusion:
Acute alcohol intake has no impact on the conversion of INH to its metabolite acetylisoniazid, which is catalysed by the enzyme N-acetyltranferase. Accordingly, a metabolic effect of acute alcohol intake on INH metabolism probably contributes little to the therapeutic failure of anti-tuberculosis treatment among alcoholics.
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