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Updated: Aug 20, 2026

Real-Time Quantification of Reactive Oxygen Species in Neutrophils Infected with Meningitic Escherichia Coli
Published on: April 20, 2021
Pyrroloquinoline-quinone: a reactive oxygen species scavenger in bacteria
Hari S Misra1, Nivedita P Khairnar, Atanu Barik
1Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400 085, India. hsmisra@magnum.barc.ernet.in
Abstract:
Transgenic Escherichia coli expressing pyrroloquinoline-quinone (PQQ) synthase gene from Deinococcus radiodurans showed superior survival during Rose Bengal induced oxidative stress. Such cells showed significantly low levels of protein carbonylation as compared to non-transgenic control. In vitro, PQQ reacted with reactive oxygen species with rate constants comparable to other well known antioxidants, producing non-reactive molecular products. PQQ also protected plasmid DNA and proteins from the oxidative damage caused by gamma-irradiation in solution. The data suggest that radioprotective/oxidative stress protective ability of PQQ in bacteria may be consequent to scavenging of reactive oxygen species per se and induction of other free radical scavenging mechanism.
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