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Updated: Aug 20, 2026

Single Oocyte Bisulfite Mutagenesis
Published on: June 27, 2012
The DNA methylation events in normal and cloned rabbit embryos
Tao Chen1, Yan-Ling Zhang, Yan Jiang
1State Key Laboratory of Reproductive Biology, Institute of Zoology, Graduate School, The Chinese Academy of Sciences, Beijing 100080, China.
Abstract:
To study the DNA methylation events in normal and cloned rabbit embryos, we investigated the methylation status of a satellite sequence and the promoter region of a single-copy gene using bisulfite-sequencing technology. During normal rabbit embryo development, both sequences maintained hypermethylation status until the 8- to 16-cell stage when progressive demethylation took place. In cloned embryos, the single-copy gene promoter sequence was rapidly demethylated and precociously de novo methylated, while the satellite sequence maintained the donor-type methylation status in all examined stages. Our results indicate that unique sequences as well as satellite sequences may have aberrant methylation patterns in cloned embryos.
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