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Related Experiment Videos

Dynamics and plasticity in developing neuronal networks in vitro.

Jaap van Pelt1, Ildiko Vajda, Pieter S Wolters

  • 1Netherlands Institute for Brain Research, Graduate School Neurosciences Amsterdam, Meibergdreef 33, 1105 AZ Amsterdam, The Netherlands. j.van.pelt@nih.knaw.nl

Progress in Brain Research
|December 8, 2004
PubMed
Summary

Developing neuronal networks in vitro exhibit evolving spontaneous firing patterns. These include network bursts with stable spatio-temporal organization and age-specific changes in firing dynamics over weeks.

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Area of Science:

  • Neuroscience
  • Developmental Neuroscience
  • Computational Neuroscience

Background:

  • Dissociated cortical tissue in culture forms neuronal networks with axonal and dendritic arborizations.
  • Developing neuronal networks in vitro exhibit spontaneous firing activity starting around the first week.
  • Multielectrode arrays allow simultaneous recording of firing activity from multiple neurons over extended periods.

Purpose of the Study:

  • To summarize recent findings on the characteristics and developmental changes in spontaneous firing dynamics of neuronal networks in vitro.
  • To investigate the relationship between neuronal development, synaptic connectivity, and firing dynamics.
  • To explore age-specific patterns and stability of network activity.

Main Methods:

  • Culturing dissociated cortical tissue on multielectrode arrays.

Related Experiment Videos

  • Simultaneous recording of neuronal firing activity over long durations.
  • Analysis of spontaneous firing dynamics, including network bursts and their spatio-temporal organization.

    • Main Results:

    • Observed long-lasting transient periods of increased firing (days to weeks) and age-specific synchronous network firing (network bursts on a seconds timescale).
    • Spatio-temporal organization within network bursts demonstrated high stability over many hours.
    • Progressive evolution noted, including broadening of burst firing rate profile (3rd week in vitro) and abrupt onset with precise spike timing (from 5th week in vitro onwards).

    Conclusions:

    • Developmental changes in spontaneous firing dynamics are linked to structural network changes and activity-dependent plasticity.
    • Network bursts exhibit stable spatio-temporal organization and evolve with age in vitro.
    • Preliminary findings suggest patterns in spike sequences within bursts and effects of external stimulation.