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Updated: Aug 20, 2026

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
Published on: September 16, 2019
Efficient cleavage of RNA, enhanced cellular uptake, and controlled intracellular localization of conjugate DNAzymes
Takanori Kubo1, Kengo Takamori, Ken-ichi Kanno
1Department of Biological and Environmental Chemistry, School of Science and Technology, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
Abstract:
Conjugate DNAzymes with polyamines and peptides were successfully prepared by solid phase fragment condensation (SPFC) and showed up to 4.2 times higher catalytic efficiency (k(cat)/K(m)) and enhanced tolerance against DNase 1digestion. To be pointed out, intracellular localization of DNAzymes could be controlled by conjugated with naturally occurring signal peptides responsible for nuclear cytoplasmic transport of proteins.
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