Steroid epimers studied by different mass spectrometric methods
Marianna Mák1, Erzsébet Francsics-Czinege, Zoltán Tuba
1Gedeon Richter Ltd., Budapest, P.O. Box 27, H-1475, Hungary. m.mak@richter.hu
Abstract:
The combined use of different mass spectrometric ionization methods and MS/MS techniques provide the possibility to differentiate between stereoisomers or epimers. In this paper the mass spectral decomposition of 11alpha- and 11beta-substituted estrans was studied. Distinctive stereochemical effects have been observed in their fast atom bombardment product ion spectra. In the electron ionisation (EI) mode, the 5alpha- and 5beta-hydroxylated compounds showed significant differences in the abundance of water elimination. Mass spectrometry has proved to be an effective tool when stereoisomer steroids are compared.
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