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Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
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Published on: January 6, 2015

Green fluorescent protein as a reporter in translational assays

Xue-Qing Wang1, Thomas Hadwen, Joseph A Rothnagel

  • 1Department of Biochemistry and Molecular Biology, The Institute for Molecular Bioscience and The Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Qld. 4072, Australia.

Analytical Biochemistry
|December 8, 2004
PubMed
Abstract

No abstract available in PubMed .

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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