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Updated: Jan 20, 2026
The ATP Bioluminescence Assay
Published on: April 30, 2023
[Changes in ultraweak luminescence, ATP and active oxygen contents during apricot florescence]
Xin-Hua Zhang1, Hong-Qiang Yang, Fu-Jun Li
1College of Horticulture, Shandong Agricultural University, Taían 271018, China.
Abstract:
Badan Shuixing apricot (Amenia cavulgaris Lam.) was used to study the changes in UWL intensity and its relationship to ATP content, respiration rate and active oxygen production rate during florescence of apricot. The results indicated that the production rate of active oxygen was smooth before the peak season of blossom, the UWL intensity and ATP content were both rise and the respiration rate also showed the trend of rise mainly at the same time. After the peak season of blossom, the respiration rate and ATP content declined rapidly while the UWL intensity went down slowly, but the active oxygen production rate increased rapidly. The protein content increased at first and then decreased during the florescence of apricot. The change in MDA content was similar to the change in active oxygen production rate, which rose rapidly after the peak season of blossom. It was concluded that the UWL was closely related to the energy level symbolized by ATP content and was affected by active oxygen metabolism in the apricot flower. The ATP content was the main factor to lead to the rise of UWL before the full bloom stage while the action of active oxygen on UWL appeared just after the peak season of blossom.
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