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Updated: Aug 20, 2026

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants
Published on: June 12, 2026
Simple high-performance liquid chromatographic method for determination of atropine and obidoxime in a parenteral
Ahmet C Gören1, Gökhan Bilsel, Mine Bilsel
1TUBITAK UME (National Metrology Institute), 41470 Gebze-Kocaeli, Turkey. ahmet.goren@ume.tubitak.gov.tr
Abstract:
Atropine and obidoxime in a parenteral injection device are determined by simple HPLC method simultaneously without any pretreatment at 228 nm. The relative standard deviations (R.S.D.) were below 1.6% for the compounds. The correlation coefficient was greater than 0.999 for both compounds in the calibration range. The recoveries at 5 mg/L concentration averaged as 95% for atropine and 102% for obidoxime. The uncertainty of the measurements for atropine and obidoxime was 2.8% and 2.4%, respectively.
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