Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preoperative Systemic Pan-Immune-Inflammation Value Predicts Posthepatectomy Liver Failure After Laparoscopic Resection for Hepatocellular Carcinoma: A Retrospective Cohort Study.

Journal of inflammation research·2026
Same author

Small Molecule Activators of Antitumor Immunity.

Accounts of chemical research·2026
Same author

Structural Control of Dual Emission in Coumarin Fluorophores for Visualizing Protein Droplet Maturation.

Angewandte Chemie (International ed. in English)·2026
Same author

National and Provincial Lifetime Risk of Chronic Respiratory Disease - China, 2023.

China CDC weekly·2026
Same author

National and regional lifetime risks of developing and dying from digestive system cancers in China: Findings of the Global Burden of Disease Study 2023.

Chinese medical journal·2026
Same author

Acellular Dermal Matrix-assisted Implant-based Breast Reconstruction: Surgical and Patient-reported Outcomes From the Mastectomy Reconstruction Outcomes Consortium Study.

Plastic and reconstructive surgery. Global open·2026

Related Experiment Video

Updated: Jul 5, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

Small molecule transcription factor mimic.

Youngjoo Kwon1, Hans-Dieter Arndt, Qian Mao

  • 1The Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.

Journal of the American Chemical Society
|December 9, 2004
PubMed
Summary
This summary is machine-generated.

Researchers created a synthetic, nonpeptidic molecule that mimics a transcription factor. This novel compound controls gene expression by binding to DNA and a protein complex, demonstrating a new approach in chemical biology.

More Related Videos

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Related Experiment Videos

Last Updated: Jul 5, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Area of Science:

  • Chemical Biology
  • Molecular Biology
  • Synthetic Chemistry

Background:

  • Transcription factors regulate gene expression, a fundamental process in eukaryotic biology.
  • External control of transcription factors using small organic molecules is a significant challenge in chemistry.

Purpose of the Study:

  • To design and synthesize a completely organic, nonpeptidic compound that mimics the function of a transcription factor.
  • To demonstrate that such a synthetic molecule can externally control gene expression.

Main Methods:

  • Designed a hybrid molecule combining a hairpin polyamide for DNA binding and wrenchnolol for binding to the Sur-2 subunit of the human mediator complex.
  • Tested the compound's ability to activate transcription of a reporter gene in vitro.

Main Results:

  • The hybrid organic compound successfully activated reporter gene transcription in a promoter-dependent manner.
  • The compound achieved this activation through simultaneous interactions with both DNA and the Sur-2 protein.

Conclusions:

  • It is feasible to engineer synthetic, nonpeptidic molecules that function as transcription factors.
  • This work opens new avenues for controlling gene expression using designed organic compounds.