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Quantitative RT-PCR assay of 5-HT1A and 5-HT2A serotonin receptor mRNAs using genomic DNA as an external standard

Alexander V Kulikov1, Vladimir S Naumenko, Irina P Voronova

  • 1Institute of Cytology and Genetics, Russian Academy of Sciences, Avenue Lavrentyev, 10 630090 Novosibirsk, Russia. akulikov@ngs.ru

Journal of Neuroscience Methods
|December 9, 2004
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...