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TERMINUS--Telomeric End-Read Mining IN Unassembled Sequences.

Weixi Li¹, Cathryn J Rehmeyer, Chuck Staben

¹Department of Biological Sciences, University of Kentucky, Lexington, KY 40546, USA.

Bioinformatics (Oxford, England)

|December 9, 2004

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TERMINUS is a novel bioinformatics tool designed to map telomeres in whole genome shotgun sequencing projects. It identifies chromosome ends, estimates telomeric gap sizes, and aids in gap closure for improved genome assembly.

Area of Science:

Genomics
Bioinformatics

Background:

Telomeres are crucial protective caps at the ends of eukaryotic chromosomes.
Mapping telomeres in draft genome sequences presents significant challenges.
Accurate telomere identification is essential for complete genome assembly.

Purpose of the Study:

To introduce TERMINUS, a computational tool for mapping telomeres.
To facilitate the identification of telomere-proximal regions in draft genomes.
To assist in estimating telomeric gap sizes and identifying clones for gap closure.

Main Methods:

Mining raw sequence reads from trace archives for telomeric reads.
Assembling telomeric reads into contigs representing chromosome ends.
BLASTing consensus sequences against genome assemblies to identify telomere-proximal contigs.

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Main Results:

TERMINUS successfully maps telomeres on draft genome sequences.
The tool estimates sizes of telomeric gaps.
It identifies specific clones required for closing these gaps.

Conclusions:

TERMINUS provides a robust method for telomere mapping in large-scale sequencing projects.
The tool aids in improving the completeness and accuracy of genome assemblies.
It offers valuable insights into chromosome end structures and organization.