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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
Published on: August 4, 2016
cDNA microarray gene expression analysis and toxicological phenotype for anticancer drug
Gyoung-Jae Lee1, Wan-Seon Lee, Ki-Seon Jeon
1Research Institute, Shin-Won Scientific Co., Ltd. Gyeonggi-do, South Korea.
Abstract:
Toxicogenomics, the subdiscipline that merges genomics with toxicology, hold the promise to contributing toward the goal of elucidating mechanism by studying genomic profiling related with various drugs. The application of gene expression profiling technology to examine multiple genes and signaling pathways promises a significant advance in understanding the toxic mechanisms of various drugs and prediction of new drug candidate. Toxicogenomics is emerging field combining genomics and bioinformatics to identify and characterize mechanisms of toxicity of drug and various compounds. The principal hypothesis underlying on this field is that chemical-specific pattern of altered gene expression is related with each chemicals properties, especially toxicological property, and it will be revealed using high-density microarray analysis of sample from exposed organisms. So, in this study we compare the gene expression pattern of two anticancer drugs paclitaxel and orally absorbable paclitaxel, using the cDNA microarray. And from the result of this study, it is possible to provide the new possibility for genome-wide insight into mechanism of their anticancer activity and toxicological phenotype.
Insights
Toxicogenomics uses gene expression to understand drug toxicity. This study compared gene patterns of paclitaxel and an oral form, revealing insights into anticancer activity and toxicity.
Area of Science:
- Toxicogenomics
- Genomics
- Bioinformatics
- Drug Discovery
Background:
- Toxicogenomics merges genomics and toxicology to elucidate drug mechanisms.
- Gene expression profiling aids in understanding drug toxicity and predicting new candidates.
- The core hypothesis is that chemical-specific gene expression patterns reveal toxicological properties.
Purpose of the Study:
- To compare gene expression patterns of paclitaxel and orally absorbable paclitaxel.
- To gain genome-wide insights into the anticancer activity and toxicological phenotype of these drugs.
Main Methods:
- Utilizing toxicogenomics principles.
- Employing gene expression profiling technology.
- Conducting cDNA microarray analysis on samples from exposed organisms.
Main Results:
- Identified and compared distinct gene expression patterns between paclitaxel and its orally absorbable form.
- Provided a genome-wide perspective on the molecular mechanisms underlying their anticancer effects and toxicity.
Conclusions:
- Gene expression profiling is a powerful tool for understanding drug mechanisms.
- This comparative analysis offers new possibilities for evaluating anticancer drug activity and toxicity.
