Related Experiment Video
Updated: Aug 20, 2026

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
Published on: August 25, 2018
[Random amplified polymorphic DNA analysis of the genomes among 7 species of ticks]
Yin-shu Yang1, Hong-bin Zhao, Jin-xue Di Wu
1Military Medical Institute of Lanzhou Military Command, Lanzhou 730020, China.
Objective:
To study genomic polymorphic DNA and genetic distance of 7 species of ticks.
Methods:
Ticks used in this study were Dermacentor nuttalli, D. silvarum, Haemaphysalis qinghaiensis, H. formosensis, H. punctata, Amblyomma testudinarium, and Ixodes ovatus. DNA extracts of the 7 species of ticks were amplified by random amplified polymorphic DNA (RAPD) and PCR technique using 5 primers with different arbitrary single chain polynucleotide sequences. DNA fingerprint maps were analyzed and the genetic distance among 7 species of ticks were counted.
Results:
The amplified products of the 7 species of ticks by RAPD all showed their specific DNA band. The average genetic distance among them was 0.71.
Conclusion:
RAPD can differentiate the 7 species of ticks.
Related Concept Videos
Modern Molecular Taxonomy
Applications of Molecular Taxonomy

