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[Immunoscreening of newborn larvae cDNA library of Trichinella spiralis]
Xiu-ping Wu1, Bao-quan Fu, Ming-yuan Liu
1Faculty of Military Veterinary, Quartermaster University of PLA, Changchun 130062, China.
Objective:
To obtain antigenic genes encoding newborn larvae antigens of Trichinella spiralis.
Methods:
Newborn larvae (NBL) cDNA library of Trichinella spiralis was screened using the infected swine serum and positive clones were sequenced and analysed.
Results:
158 positive clones were obtained from 4.5 x 10(5) recombinant clones by immunoscreening. The sequence analysis of positive clones showed that there are 36 novel cDNAs, 5 reported cDNAs and 12 cDNAs without the ORF showing high similarity to the mitochondrial DNA of Trichinella spiralis.
Conclusion:
Some cDNAs encoding antigenic protein of newborn larvae of Trichinella spiralis were obtained.
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