Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA repair: keeping it together.

Michael Lisby1, Rodney Rothstein

  • 1Institute of Molecular Biology, University of Copenhagen, Øster Farimagsgade 2A, DK-1353 Copenhagen K, Denmark. mlisby@my.molbio.ku.dk

Current Biology : CB
|December 14, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multi-species toolkit of <i>TOP2</i> hypercleavage mutants for studying topoisomerase II-mediated DNA damage.

bioRxiv : the preprint server for biology·2026
Same author

TOPBP1-PLK1 interaction preserves genome integrity during mitosis.

Nucleic acids research·2026
Same author

Dss1 facilitates Rad51 recruitment downstream of BRCA2/Brh2 in response to DNA damage.

DNA repair·2026
Same author

Stepwise DNA damage and repair mechanisms at replication forks in response to topoisomerase I inhibition.

DNA repair·2025
Same author

Click-Cyclized Cell Penetrating Peptides Containing Hydrophobic Proline Derivatives for Efficient Intracellular Delivery.

Angewandte Chemie (International ed. in English)·2025
Same author

TopBP1 coordinates DNA repair synthesis in mitosis via recruitment of the nuclease scaffold SLX4.

Communications biology·2025

A novel protein scaffold stabilizes chromosomes during DNA double-strand breaks. This crucial structure, reliant on Rad52 and the Rad50-Mre11-Xrs2 complex, resists mitotic spindle forces during cell cycle arrest.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • DNA double-strand breaks (DSBs) are severe DNA lesions that can lead to genomic instability.
  • Cellular mechanisms are essential for repairing DSBs and maintaining chromosome integrity during cell division.
  • The DNA damage checkpoint ensures proper repair before cell cycle progression.

Discussion:

  • A newly identified protein scaffold plays a critical role in holding chromosomes together when DSBs occur.
  • This scaffold's function is dependent on the Rad52 protein and the conserved Rad50-Mre11-Xrs2 complex.
  • The scaffold demonstrates remarkable resilience, withstanding the mechanical forces exerted by the mitotic spindle.

Key Insights:

  • Discovery of a protein scaffold essential for chromosome stability following DNA double-strand breaks.

Related Experiment Videos

  • Elucidation of the scaffold's dependence on key DNA repair proteins: Rad52 and the Rad50-Mre11-Xrs2 complex.
  • Demonstration of the scaffold's ability to resist physical forces during mitotic arrest.

    • Outlook:

    • Further investigation into the precise structural and mechanical properties of the protein scaffold.
    • Exploring the potential therapeutic implications of targeting this scaffold in cancer or genetic disorders.
    • Understanding the interplay between the scaffold, DNA repair pathways, and mitotic progression.