Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simplifying DOSY spectra with selective TOCSY edited preparation.

Scott A Bradley1, Krish Krishnamurthy, Haitao Hu

  • 1Drug Disposition, Lilly Research Laboratories, Indianapolis, IN 46285, USA.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|December 14, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effect of Water-Solid Ratio on the Performance, Microstructure Evolution, and Low-Carbon Characteristics of Multi-Solid-Waste-Based Flowable Stabilized Soil.

Materials (Basel, Switzerland)·2026
Same author

Engineering Long-Term Hydrogel Stability for Reliable Bioelectronics.

ACS nano·2026
Same author

Effects of Prolonged Cryogenic Exposure on the Electrical Degradation of Stator Main Insulation in Wind Turbines.

Materials (Basel, Switzerland)·2026
Same author

Solidification Performance and Mechanism of TSC Composite Soil Based on Microbially Induced Mineralization.

Materials (Basel, Switzerland)·2026
Same author

Development and validation of a novel prognostic model for gastric signet ring cell carcinoma based on inflammation-nutrition indicators.

Frontiers in nutrition·2026
Same author

Clinical utility of fecal Sanger sequencing and PCR alongside gastric biopsy E-test for <i>Helicobacter pylori</i> resistance to clarithromycin and quinolone.

Microbiology spectrum·2026

This study introduces a novel Diffusion-Ordered NMR Spectroscopy (DOSY) method using selective TOCSY to resolve overlapped signals. The technique effectively simplifies complex spectra by transferring magnetization, improving analyte signal clarity.

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Nuclear Magnetic Resonance

Background:

  • Diffusion-Ordered NMR Spectroscopy (DOSY) is powerful but limited by signal overlap in proton spectra.
  • Resolving signals in complex mixtures, like sucrose/raffinose, remains a challenge for standard DOSY.

Purpose of the Study:

  • To develop an improved DOSY experiment for resolving severely overlapped signals.
  • To enhance spectral resolution in complex NMR samples.

Main Methods:

  • A DOSY experiment incorporating selective Total Correlation Spectroscopy (TOCSY) as a preparation period.
  • Utilizing a zero-quantum filter within the selective TOCSY to prevent coherence transfer.
  • Expanding the method into a 3D sequence with BASHD-TOCSY for homonuclear decoupling.

Related Experiment Videos

Main Results:

  • Selective TOCSY enabled excitation of resolved signals and magnetization transfer to buried resonances.
  • The zero-quantum filter effectively prevented unwanted coherence from entering the DOSY sequence.
  • Both 2D and 3D sequences significantly simplified DOSY spectra of a 'dirty' sucrose/raffinose mixture.
  • The 3D sequence achieved complete resolution of target signals, eliminating matrix background interference.

Conclusions:

  • The proposed selective TOCSY-edited DOSY method overcomes limitations of traditional DOSY for overlapped signals.
  • The 3D extension offers superior spectral resolution and simplifies analysis of complex mixtures.
  • This technique provides a powerful tool for analyzing challenging NMR samples.