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Plant transformation: a simple particle bombardment device based on flowing helium
Y Takeuchi1, M Dotson, N T Keen
1Department of Plant Pathology, University of California, Riverside 92521.
Plant Molecular Biology
|February 1, 1992
Abstract:
We observed that flowing helium at moderate pressures accelerated DNA-coated microprojectiles to velocities suitable for penetration of cells in intact plant tissues. The flowing helium principle permitted the construction of a simple and inexpensive transformation device that was easier to use than those previously described. This device provided efficient transformation of cells in soybean seedlings and other plants.