Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The neuronal calcium-sensor proteins.

Robert D Burgoyne1

  • 1The Physiological Laboratory, School of Biomedical Sciences, University of Liverpool, Crown Street, Liverpool, L69 3BX, UK.

Biochimica Et Biophysica Acta
|December 14, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lysosome exocytosis is required for mitosis in mammalian cells.

Biochemical and biophysical research communications·2022
Same author

A centrosome-localized calcium signal is essential for mammalian cell mitosis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2019
Same author

Calcium Sensors in Neuronal Function and Dysfunction.

Cold Spring Harbor perspectives in biology·2019
Same author

A Caenorhabditis elegans assay of seizure-like activity optimised for identifying antiepileptic drugs and their mechanisms of action.

Journal of neuroscience methods·2018
Same author

α-Methyl-α-phenylsuccinimide ameliorates neurodegeneration in a C. elegans model of TDP-43 proteinopathy.

Neurobiology of disease·2018
Same author

Ethanol Stimulates Locomotion via a G<sub>αs</sub>-Signaling Pathway in IL2 Neurons in <i>Caenorhabditis elegans</i>.

Genetics·2017
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

Intracellular calcium (Ca2+) concentration changes regulate neuronal function via Ca2+-binding proteins. The neuronal calcium sensor (NCS) family, particularly those with Ca2+/myristoyl switches, plays key roles in neurons.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Intracellular free Ca(2+) concentration ([Ca(2+)](i)) is a critical regulator of neuronal function, influencing processes from rapid ion channel modulation to long-term gene expression.
  • Ca(2+) effects are mediated by Ca(2+)-binding proteins that act as sensors, undergoing conformational changes upon Ca(2+) binding to modulate target proteins.

Purpose of the Study:

  • To explore the role of the neuronal calcium sensor (NCS) protein family in neuronal function.
  • To investigate the structural and functional characteristics of NCS proteins, including their membrane targeting mechanisms.

Main Methods:

  • Analysis of the 14-member NCS protein family in mammalian species.
  • Examination of N-terminal myristoylation and the Ca(2+)/myristoyl switch mechanism.

Related Experiment Videos

  • Review of molecular genetic analyses confirming functional roles.

    • Main Results:

    • NCS proteins are primarily expressed in photoreceptor cells and neurons.
    • Many NCS proteins utilize N-terminal myristoylation for membrane association, with some exhibiting a Ca(2+)-dependent exposure of the myristoyl group (Ca(2+)/myristoyl switch).
    • NCS proteins are implicated in diverse neuronal regulatory functions.

    Conclusions:

    • The NCS protein family is crucial for translating Ca(2+) signals into cellular responses within neurons.
    • The Ca(2+)/myristoyl switch is a significant mechanism for regulating NCS protein localization and function.
    • Further molecular genetic studies have validated the involvement of NCS proteins in various neuronal processes.