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Related Experiment Videos

c-kit and ureteral peristalsis.

Scott G David1, Cristina Cebrian, E Darracott Vaughan

  • 1Department of Urology, New York Presbyterian Hospital-Cornell Medical Center and Department of Physiology, Weill Medical College of Cornell University, New York, NY, USA.

The Journal of Urology
|December 14, 2004
PubMed
Summary

The c-kit receptor tyrosine kinase is newly expressed in embryonic mouse ureters and is essential for ureteral peristalsis during development. This finding is crucial for understanding urinary tract development and function.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Urology

Background:

  • c-kit, a tyrosine kinase receptor, is vital for embryonic cell differentiation, including gut pacemaker cells.
  • Functional c-kit is necessary for gut peristalsis.
  • c-kit expression is present in the adult murine urinary tract.

Purpose of the Study:

  • To investigate the temporal expression of c-kit during ureter development in vivo.
  • To determine if c-kit activity is essential for ureteral peristalsis in vitro.

Main Methods:

  • Immunocytochemistry was used to analyze c-kit, alpha-smooth muscle actin, and uroplakin expression in murine kidneys and ureters (gestational days 12.5-17.5).
  • Ureters from gestational day 15.5 mice were cultured in vitro with or without c-kit inhibiting antibodies.

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Main Results:

  • c-kit expression was detected in renal epithelia and ureteropelvic adventitia by gestational day 15.5, with prominent staining in the proximal ureter's muscularis propria.
  • In vitro, embryonic ureters developed unidirectional contractions after 3 days of culture, coinciding with c-kit upregulation.
  • Inhibiting c-kit activity in cultured ureters significantly altered their morphology and peristalsis.

Conclusions:

  • Initial c-kit expression and localization in the embryonic murine upper urinary tract were identified.
  • c-kit expression increases in the developing ureter before its ability to contract unidirectionally.
  • c-kit function is required for embryonic ureteral peristalsis in vitro.