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Identifying citrus limonoid aglycones by HPLC-EI/MS and HPLC-APCI/MS techniques
Gary D Manners1, Andrew P Breksa
1United States Department of Agriculture, Agricultural Research Service, Western Regional Research Center, 800 Buchanan St. Albany, CA 94710, USA. gmann@pw.usda.gov
Abstract:
HPLC coupled with normal phase electron ionisation (EI) and atmospheric pressure chemical ionisation (APCI)/ mass spectrometry methods has been applied to identify 17 known neutral limonoid aglycones from Citrus sources. The HPLC-MS data from the known limonoids provided chromatographic characteristics, APCI-derived molecular weight data and EI fragmentation data for each limonoid. EI fragmentation patterns for the limonoids were correlated with structural characteristics. The EI fragmentation patterns coupled with APCI-derived molecular weights were utilised as a potential method by which to discern the structural character of unknown citrus limonoids.
