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Updated: Aug 20, 2026
Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Determination of alpha-tocopherolquinone in human serum samples by liquid chromatography with fluorescence detection
Dagmar Pollok1, Hans-Ulrich Melchert
1Robert Koch-Institute, Pharmacoepidemiology, Seestr. 10, 13353 Berlin, Germany.
Abstract:
A HPLC/fluorescence method with on-line post-column derivatization by a photoreactor was developed, where alpha-, beta + gamma- and delta-tocopherolquinone (TQ) are separated on a 250 mm x 4.6 mm RP-18 column. The LOD is about 250 pg for all TQs. In combination with a two-step sample preparation procedure, this method was successfully employed for measurement of alpha-TQ in human serum samples. Recovery for alpha-TQ from spiked serum was excellent (99 +/- 5%) and results of alpha-TQ determinations in 111 serum samples are reported. Additionally, possibilities for determination of other TQs in serum and alternative derivatization with a zinc reduction column are discussed.