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Updated: Aug 20, 2026

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
Published on: August 30, 2018
Hydrophilic interaction chromatography combined with tandem-mass spectrometry to determine six aminoglycosides in
R Oertel1, V Neumeister, W Kirch
1Institute of Clinical Pharmacology, Medical Faculty Carl Gustav Carus, Technical University Dresden, FiedlerStrasse 27, D-01307 Dresden, Germany. reioer@rcs.urz.tu-dresden.de
Abstract:
A specific and automated method was developed to quantitate the aminoglycosides amikacin, gentamicin, kanamycin, neomycin, paromomycin, and tobramycin simultaneously in human serum. Samples were prepared with an automated solid phase extraction (SPE). The hydrophilic interaction chromatography (HILIC) was used for separation of analytes from endogenous compounds and baseline separation. The aminoglycosides were detected with electrospray ionisation tandem mass spectrometry (ESI-MS-MS). Using a volume of 500 microl biological sample the lower limits of quantification were 100 ng/ml or better. The described HILIC-MS-MS method is suitable for therapeutic drug monitoring and for clinical and pharmcokinetical investigations of the aminoglycosides.
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