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Assignment of SRY, ANT3, and CSF2RA to the bovine Y chromosome by FISH and RH mapping
Wan-Sheng Liu1, F Abel Ponce de León
1Department of Animal Biotechnology, College of Agriculture, Biotechnology, and Natural Resources, University of Nevada, Reno, Nevada 89557, USA. wsliu@cabnr.unr.edu
Abstract:
Three genes, SRY, ANT3, and CSF2RA, were mapped to the bovine Y chromosome (BTAY) by fluorescence in situ hybridization (FISH) and/or radiation hybrid (RH) mapping. FISH analysis indicated that the bovine SRY gene maps to the distal region of BTAYq, while ANT3 and CSF2RA are located in the pseudoautosomal region (PAR) of BTAYp and BTAXq. RH mapping with a 7000-rad cattle hamster whole-genome radiation hybrid panel further defined the ANT3 and CSF2RA position in relationship to previously mapped 12 PAR markers, and resulted in a relatively high resolution RH map for the PAR of BTAY.

