Related Experiment Video
Updated: Aug 20, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
Published on: May 17, 2016
Myostatin dominant negative allele products interact positively with wild type monomers
Cristina Fernandez1, Angel Barroso, Javier Cañón
1Facultad de Veterinaria, Dpto de Producción Animal, Laboratorio de Genética Molecular, Universidad Complutense, Madrid, Spain.
Abstract:
Myostatin is an extracellular negative regulator of muscle growth with an important role in bovine muscular hypertrophy. It belongs to the transforming growth factor beta (TGFbeta) superfamily, and has structural and functional characteristics similar to those of its other, members. Based on these characteristics, we designed three gene constructs in order to create a series of dominant negative (DN) alleles for murine myostatin. As a first requirement for any DN strategy, we first showed that each of the three mutant DN monomers were able to interact with wild type mature myostatin (wt-Mstn), both in a pull-down and a mammalian two-hybrid assay. In addition, the degree of DN-Mstn/wt-Mstn interaction was similar to that of wt-Mstn/wt-Mstn. These results suggest that the three designed alleles are good candidates for use in a DN-based strategy for generating muscular hypertrophy in cattle.
Related Concept Videos
Satellite Stem Cells and Muscular Dystrophy
Formation of Muscle Fibers from Myoblasts
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
Cytoskeletal Accessory Proteins
Actin Polymerization and Cell Motility
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate.
Mutations
The Sarcomere
Each myosin...

