Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recombinant cytochrome p450 immobilization for biosensor applications.

Cristina Paternolli1, Mirco Antonini, Paola Ghisellini

  • 1Nanoworld Institute and Biophysics Division, University of Genova, Corso Europa, 30, 16132 Genova, Italy.

Langmuir : the ACS Journal of Surfaces and Colloids
|December 15, 2004
PubMed
Summary

Researchers developed optimal biodevices using P450 cytochrome enzymes for sensing organic compounds. Langmuir-Blodgett films provided stable conditions for detecting clozapine, styrene, and cholesterol.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Dual Cell-Line Molecular Platform to Assess Sub-Threshold Biocompatibility: Application to Processed Orthodontic Clears Aligners.

Orthodontics & craniofacial research·2026
Same author

Comparative Evaluation of qPCR and Digital PCR for the Detection of <i>Nosema ceranae</i> in Honey Bees.

Veterinary sciences·2025
Same author

Molecular Biocompatibility Assessment of PETG Aligners After Processing by Laser or Milling.

Materials (Basel, Switzerland)·2025
Same author

A Novel Biosensor for the Early Detection of <i>Aethina tumida</i> via <i>Kodamaea ohmeri</i> in Honeybee Colonies.

Insects·2025
Same author

Evaluation of rapid detection methods for H5N1 virus using biosensors: An AI-based study.

Bioinformation·2025
Same author

Evaluation of estrogenic and anti-estrogenic activity of endocrine disruptors using breast cancer spheroids: a comparative study of T47D and MCF7 cell lines in 2D and 3D models.

Frontiers in toxicology·2025

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Biosensors

Background:

  • Heme-enzymes, specifically P450 cytochrome isoforms, possess attractive pleiotropic properties.
  • Developing sensitive and selective biosensors for organic substances is crucial for various applications.

Purpose of the Study:

  • To establish a general optimal procedure for biodevice assembly using P450 cytochromes for sensing diverse organic substances.
  • To investigate the efficacy of different immobilization techniques for enhanced sensor performance.

Main Methods:

  • Utilized three P450 cytochrome isoforms (P4501A2, P4502B4, P450SCC).
  • Employed Langmuir-Blodgett films for protein immobilization.
  • Characterized nanostructures using UV-vis spectrophotometry, nanogravimetry, circular dichroism, and electrochemical methods.

Related Experiment Videos

  • Investigated the use of a gel-matrix for low-purity protein immobilization.

    • Main Results:

    • Langmuir-Blodgett films provided optimal stable working conditions for P450 cytochromes.
    • Ordered nanostructures were identified as optimal for sensing clozapine, styrene, and cholesterol.
    • A gel-matrix was necessary for optimal clozapine sensing with low-purity P4501A2.
    • P450-based sensors demonstrated capability in detecting fatty acids, drugs, and toxic compounds.

    Conclusions:

    • Optimized biodevice assembly using P450 cytochromes and Langmuir-Blodgett films enables sensitive detection of various organic compounds.
    • Proper immobilization and nanostructured mutants lead to stable and selective P450-based sensors.
    • These biosensors hold potential for detecting a wide range of organic substrates, including pharmaceuticals and environmental toxins.