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Updated: Aug 14, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
Published on: March 20, 2015
Internal standard in surface-enhanced Raman spectroscopy
A Lorén1, J Engelbrektsson, C Eliasson
1Analytical and Marine Chemistry, Department of Chemistry and Bioscience, Chalmers University of Technology, SE-412 96 Göteborg, Sweden.
Abstract:
A method is presented for the use of SAM layers as internal standards for calibration in surface-enhanced Raman spectroscopy. Three cyano-containing compounds were attached to gold colloids via a metal-sulfur bond and evaluated for spectral stability and normalization capacity. The results show that the analyte, rhodamine 6G, and the internal standard signal enhancement covaried, and it was possible to quantify the analyte with PLS. The fact that the enhancing substrate was chaotic assemblies with large variation in signal enhancement shows the versatility of this method.
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