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Related Experiment Videos

Internal standard in surface-enhanced Raman spectroscopy.

A Lorén1, J Engelbrektsson, C Eliasson

  • 1Analytical and Marine Chemistry, Department of Chemistry and Bioscience, Chalmers University of Technology, SE-412 96 Göteborg, Sweden.

Analytical Chemistry
|December 15, 2004
PubMed
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Self-assembled monolayer (SAM) layers serve as effective internal standards for calibrating surface-enhanced Raman spectroscopy (SERS). This method enables reliable quantification of analytes like rhodamine 6G, even on variable substrates.

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Materials Science

Background:

  • Surface-enhanced Raman spectroscopy (SERS) is a powerful technique for detecting analytes at low concentrations.
  • Accurate quantification in SERS can be challenging due to variations in substrate enhancement.
  • Internal standards are needed for reliable calibration and normalization of SERS signals.

Purpose of the Study:

  • To develop and evaluate self-assembled monolayer (SAM) layers as internal standards for SERS.
  • To assess the spectral stability and normalization capacity of SAMs.
  • To demonstrate the versatility of this calibration method for analyte quantification.

Main Methods:

  • Three cyano-containing compounds were synthesized and attached to gold colloids via metal-sulfur bonds.

Related Experiment Videos

  • These SAMs were used as internal standards in SERS experiments.
  • Analyte (rhodamine 6G) and internal standard signals were measured.
  • Partial least squares (PLS) regression was employed for quantification.

    • Main Results:

    • The signal enhancement of the analyte and the internal standard covaried effectively.
    • The SAMs demonstrated good spectral stability and normalization capacity.
    • Accurate quantification of rhodamine 6G was achieved using PLS.
    • The method proved versatile across substrates with varying signal enhancement.

    Conclusions:

    • SAM layers are a robust and versatile internal standard for SERS calibration.
    • This approach facilitates reliable and accurate quantification of analytes.
    • The method's effectiveness is demonstrated even with heterogeneous SERS substrates.