Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Internal standard in surface-enhanced Raman spectroscopy.

A Lorén1, J Engelbrektsson, C Eliasson

  • 1Analytical and Marine Chemistry, Department of Chemistry and Bioscience, Chalmers University of Technology, SE-412 96 Göteborg, Sweden.

Analytical Chemistry
|December 15, 2004
PubMed
Summary

Self-assembled monolayer (SAM) layers serve as effective internal standards for calibrating surface-enhanced Raman spectroscopy (SERS). This method enables reliable quantification of analytes like rhodamine 6G, even on variable substrates.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regenerative Response 35 Days After Esophageal Replacement in a Porcine Model; Technical Difficulties and Attempts to Achieve Optimal Tissue Remodeling.

Artificial organs·2025
Same author

Schooling experiences in children with long-gap esophageal atresia compared with children with esophageal atresia and primary anastomosis: a Swedish study.

Orphanet journal of rare diseases·2023
Same author

Robot-assisted nephroureterectomy for upper tract urothelial carcinoma-feasibility and complications: a single center experience.

Scandinavian journal of urology·2022
Same author

Correction: Hematopoietic cell transplant in pediatric acute myeloid leukemia after similar upfront therapy; a comparison of conditioning regimens.

Bone marrow transplantation·2021
Same author

Hematopoietic cell transplant in pediatric acute myeloid leukemia after similar upfront therapy; a comparison of conditioning regimens.

Bone marrow transplantation·2021
Same author

Value of flow cytometry for MRD-based relapse prediction in B-cell precursor ALL in a multicenter setting.

Leukemia·2020

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Materials Science

Background:

  • Surface-enhanced Raman spectroscopy (SERS) is a powerful technique for detecting analytes at low concentrations.
  • Accurate quantification in SERS can be challenging due to variations in substrate enhancement.
  • Internal standards are needed for reliable calibration and normalization of SERS signals.

Purpose of the Study:

  • To develop and evaluate self-assembled monolayer (SAM) layers as internal standards for SERS.
  • To assess the spectral stability and normalization capacity of SAMs.
  • To demonstrate the versatility of this calibration method for analyte quantification.

Main Methods:

  • Three cyano-containing compounds were synthesized and attached to gold colloids via metal-sulfur bonds.

Related Experiment Videos

  • These SAMs were used as internal standards in SERS experiments.
  • Analyte (rhodamine 6G) and internal standard signals were measured.
  • Partial least squares (PLS) regression was employed for quantification.

    • Main Results:

    • The signal enhancement of the analyte and the internal standard covaried effectively.
    • The SAMs demonstrated good spectral stability and normalization capacity.
    • Accurate quantification of rhodamine 6G was achieved using PLS.
    • The method proved versatile across substrates with varying signal enhancement.

    Conclusions:

    • SAM layers are a robust and versatile internal standard for SERS calibration.
    • This approach facilitates reliable and accurate quantification of analytes.
    • The method's effectiveness is demonstrated even with heterogeneous SERS substrates.