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Conjugation of antibodies to horseradish peroxidase
1School of Biology and Biochemistry, The Queen's University, Belfast, Northern Ireland, UK.
Abstract:
Horseradish peroxidase is coupled to IgG antibody in a two-step procedure. In the first step monosaccharide residues in the enzyme are oxidized with periodate to produce aldehyde groups. Then, in the second step, the aldehyde groups are allowed to react with amino groups in the IgG antibody. The Schiff bases formed are reduced and the conjugate is purified by gel filtration.
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