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Dual-antibody sandwich enzyme-linked immunosorbent assay

William Jordan1

  • 1Department of Immunology, University of Medicine and Dentistry of New Jersey, Newark, NJ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 15, 2004
PubMed

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Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.