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Polar stationary phases for capillary electrochromatography
Chuanhui Xie1, Hongjing Fu, Jiwei Hu
1National Chromatographic R&A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, China.
Electrophoresis
|December 15, 2004
Summary
This review explores polar stationary phases for capillary electrochromatography, offering unique selectivity for polar and charged compounds. These phases differ significantly from reversed-phase methods, enabling new separation possibilities.
Area of Science:
- Analytical Chemistry
- Separation Science
Background:
- Capillary electrochromatography (CEC) is a powerful separation technique.
- Reversed-phase stationary phases are widely used but have limitations for polar analytes.
Purpose of the Study:
- To review various polar stationary phases used in capillary electrochromatographic separations.
- To compare the selectivity of polar versus reversed-phase stationary phases.
- To discuss retention mechanisms and applications of polar stationary phases.
Main Methods:
- Literature review of polar stationary phases in CEC.
- Analysis of retention selectivity for polar and charged analytes.
- Presentation of electrochromatographic separation examples.
Main Results:
- Polar stationary phases offer distinct retention selectivity compared to reversed-phase.
- Different types of polar stationary phases exhibit varied retention mechanisms.
- Successful separations of polar solutes, peptides, and basic pharmaceuticals were demonstrated.
Conclusions:
- Polar stationary phases provide alternative and complementary selectivity in CEC.
- They are effective for separating challenging polar and charged analytes.
- Further development and application of polar stationary phases in CEC are warranted.
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