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Updated: Aug 20, 2026

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification
Published on: May 5, 2017
[Antifreeze proteins and molecular genetic improvement in freezing resistance of plants]
Shan-Zhi Lin1, Zhi-Yi Zhang, Yuan-Zhen Lin
1Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, Beijing Forestry University, Beijing 100083, China. linsz2002@163.com
Abstract:
Over the past several years, the proteins and genes associated with freezing resistance and molecular genetic improvement in freezing resistance of plants have been widely studied. The recent progress of research made at home and abroad on low-temperature-induced proteins and antifreeze proteins (AFPs) with thermal hysteresis (THA) and the identification and expression regulation of low-temperature-induced genes are reviewed in this paper. Recent advances in the approaches of gene engineering that have been successfully taken in the molecular improvement of plant freezing resistance are also reviewed. Emphasis is placed on the transformation and expression of the freezing resistance genes cloned from overwintering insects, polar fishes and plant materials. Finally, some unsolved problems and the trend of research on physiological function and mechanism of AFPs, and the application of modern biotechnology to molecular improvement in freezing resistance of plants are discussed.
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