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Programmed cell death in wheat during starchy endosperm development.

Rui Li1, Sheng-Yin Lan, Zhen-Xiu Xu

  • 1College of Life Sciences, Huazhong Agricultural University, Wuhan 430070, China.

Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao = Journal of Plant Physiology and Molecular Biology
|December 16, 2004
PubMed
Summary
This summary is machine-generated.

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Wheat endosperm cells undergo a unique programmed cell death (PCD), characterized by DNA fragmentation and distinct ultrastructural changes. This PCD process differs from typical apoptosis, with cells remaining functional until fully filled with reserves.

Area of Science:

  • Plant Biology
  • Cell Biology
  • Biochemistry

Background:

  • Wheat starchy endosperm development involves programmed cell death (PCD).
  • Understanding the molecular and ultrastructural mechanisms of PCD in plant storage tissues is crucial.

Purpose of the Study:

  • To investigate the characteristics of PCD in developing wheat starchy endosperm.
  • To compare wheat endosperm PCD with typical apoptosis in other systems.

Main Methods:

  • Agarose gel electrophoresis to detect DNA fragmentation.
  • Ethylene and abscisic acid (ABA) treatments to study PCD regulation.
  • Transmission electron microscopy for ultrastructural analysis.

Main Results:

  • Wheat endosperm PCD exhibited internucleosomal DNA fragmentation (180-200 bp fragments).

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  • Ethylene accelerated DNA fragmentation; ABA reduced its extent but did not delay onset.
  • Unique ultrastructural features included chromatin aggregation, early nuclear degeneration, and continued cytoplasmic activity post-nuclear disintegration.
  • No apoptotic bodies were observed; dead cells served as nutrient reservoirs.

    • Conclusions:

    • Wheat endosperm PCD is a specialized form of cell death.
    • The process involves distinct morphological and regulatory features compared to animal apoptosis.
    • The starchy endosperm cell death strategy supports efficient nutrient accumulation.