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The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform
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Gold and silver nanoparticles functionalized with known numbers of oligonucleotides per particle for DNA detection

Yang Chen1, Jenny Aveyard, Robert Wilson

  • 1Department of Chemistry and Centre for Bioarray Innovation, Liverpool University, Liverpool, UK L69 7ZD.

Chemical Communications (Cambridge, England)
|December 16, 2004
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...