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Protein damage in drop-on-demand printers.

Gary M Nishioka1, Andrea A Markey, Charles K Holloway

  • 1H & N Instruments, Inc., PO Box 4338, Newark, Ohio 43055, USA. HNI@Infinet.com

Journal of the American Chemical Society
|December 17, 2004
PubMed
Summary

Inkjet printing can damage enzymes like peroxidase due to rapid compression. Adding sugars such as trehalose and glucose to the solution helps protect the enzyme during this process.

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Area of Science:

  • Biotechnology
  • Biochemistry
  • Materials Science

Background:

  • Inkjet printing is a versatile method for synthesizing microarrays.
  • Understanding the impact of printing on biomolecules is crucial for microarray applications.

Purpose of the Study:

  • To investigate the damage inflicted on a model enzyme, peroxidase, during inkjet printing.
  • To evaluate the protective effects of specific additives on enzyme integrity.

Main Methods:

  • Utilized inkjet printing technology to deposit solutions containing peroxidase.
  • Characterized enzyme damage through analytical methods (details not specified in abstract).
  • Assessed the impact of trehalose and glucose on enzyme stability post-printing.

Main Results:

  • Inkjet printing process causes significant damage to peroxidase.
  • The addition of trehalose and glucose demonstrably mitigates this printing-induced damage.
  • Sugars act as protective agents for the enzyme during the compression phase of printing.

Conclusions:

  • Inkjet printing poses a risk to enzyme stability in microarrays.
  • Trehalose and glucose are effective excipients for preserving enzyme function in printed microarrays.
  • This finding has implications for developing more robust inkjet-printed enzyme-based biosensors and diagnostic tools.

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