Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Extracting DNA twist rigidity from experimental supercoiling data.

Sébastien Neukirch1

  • 1Institute for Mathematics B, Swiss Federal Institute of Technology, Lausanne, Switzerland.

Physical Review Letters
|December 17, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Young's modulus of multinanolayer polymer films: the role of the interfaces.

Soft matter·2025
Same author

Traveling Perversion as Constant Torque Actuator.

Physical review letters·2023
Same author

Extraction of Silicone Uncrosslinked Chains at Air-Water-Polydimethylsiloxane Triple Lines.

Langmuir : the ACS journal of surfaces and colloids·2018
Same author

Capillarity-induced folds fuel extreme shape changes in thin wicked membranes.

Science (New York, N.Y.)·2018
Same author

Auxiliary soft beam for the amplification of the elasto-capillary coiling: Towards stretchable electronics.

Advances in colloid and interface science·2017
Same author

Drop-on-coilable-fibre systems exhibit negative stiffness events and transitions in coiling morphology.

Soft matter·2017

We modeled supercoiled DNA to understand its response to twisting. Our findings reveal DNA

Area of Science:

  • Biophysics
  • Molecular Biology
  • Polymer Physics

Background:

  • Supercoiled DNA plays a crucial role in cellular processes.
  • Understanding DNA's mechanical properties is essential for molecular biology.
  • Previous models have limitations in capturing DNA's complex behavior.

Purpose of the Study:

  • To investigate the mechanical response of supercoiled DNA using an elastic rod model.
  • To quantitatively reproduce experimental data on DNA extension versus rotation.
  • To determine key physical parameters of DNA, such as twist rigidity.

Main Methods:

  • Utilized an elastic rod model incorporating contact mechanics.
  • Analyzed extension versus rotation diagrams of single supercoiled DNA molecules.

Related Experiment Videos

  • Integrated experimental data to validate and refine the model.

    • Main Results:

    • Successfully reproduced the supercoiling response of overtwisted DNA.
    • Estimated the effective supercoiling radius of DNA.
    • Quantified the twist rigidity of B-DNA.

    Conclusions:

    • The elastic rod model accurately describes DNA supercoiling.
    • DNA twist rigidity is sensitive to salt buffer conditions.
    • Further research is needed to fully understand DNA-buffer interactions.