Endocrine responses in cows milked by hand and machine

Plasma concentrations of oxytocin, prolactin, and cortisol were compared in five Swedish Red and White cows milked by hand versus machine. Cows were divided into two groups. One group was hand-milked; the other group was machine-milked. Treatments were switched every other day. The experiment was carried out for 6 d. Blood samples were taken prior to, during, and after milking and were assayed for hormones. More oxytocin and prolactin were released in hand-milked cows. There were no significant diurnal differences between the total amount of oxytocin released for the different treatments, but prolactin tended to be higher during hand-milking in the evening than in the morning milking. Cortisol concentrations were greater during hand-milking than during machine-milking. There were no significant treatment differences with regard to the total amount of cortisol released. During morning milking, cortisol concentrations were higher during hand-milking than during machine-milking. Our data show that hand-milking results in a pronounced and prolonged release of oxytocin and prolactin.