Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Partial hepatectomy induced liver proteome changes in mice.

Christoph W Strey1, Mike S Winters, Maciej M Markiewski

  • 1Department of Pathology & Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Proteomics
|December 17, 2004
PubMed
Summary

This study used proteomics to identify proteins that change during liver regeneration after partial hepatectomy in mice. It found twelve proteins, including adipose differentiation-related protein, were upregulated, suggesting impacts on signaling and metabolism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficacy of the "critical view/open book" concept for implementing standardized right hemicolectomy with complete mesocolic excision-results from a series of minimally invasive surgical training courses.

Surgical endoscopy·2026
Same author

New Analogs of the Compstatin Family of Clinical Complement Inhibitors with Low Picomolar Target Affinity.

Journal of medicinal chemistry·2026
Same author

[The Dynamics of the German Healthcare System Require Rapid Team Development - Colorectal Fast Track Implementation as a Prototype with a Steep Learning Curve].

Zentralblatt fur Chirurgie·2025
Same author

Clinical C3 Inhibition With AMY-101 Reveals Novel Insights Into IL-8-Driven Inflammation in COVID-19.

Immunology·2025
Same author

'Complement-ing' tissue inflammation via granzyme K?

Nature immunology·2025
Same author

Cp40-mediated complement C3 inhibition dampens inflammasome activation and inflammatory mediators storm induced by Bitis arietans venom.

International immunopharmacology·2025

Area of Science:

  • Proteomics and Molecular Biology
  • Hepatology and Regenerative Medicine

Background:

  • Liver regeneration is crucial for treating liver diseases, but therapeutic strategies are limited.
  • Understanding the complex molecular mechanisms of liver regeneration is essential for identifying new therapeutic targets.

Purpose of the Study:

  • To employ a broad-range proteomic approach to simultaneously analyze signaling and metabolic pathways during liver regeneration.
  • To identify key proteins involved in the liver's regenerative process following partial hepatectomy.

Main Methods:

  • Analysis of protein expression changes in mouse livers at 6 and 12 hours post-partial hepatectomy.
  • Utilized two-dimensional gel electrophoresis and mass spectrometry (MS) for protein identification and quantification.
  • Compared protein expression against sham-operated and untreated control groups.

Related Experiment Videos

Main Results:

  • Twelve proteins were identified as significantly upregulated (≥ 2-fold) after partial hepatectomy.
  • Upregulated proteins include adipose differentiation-related protein, gamma-actin, enoyl coenzyme A hydratase 1, serum amyloid A, and eukaryotic translation initiation factor 3.
  • These findings indicate that liver regeneration impacts diverse signaling and metabolic pathways.

Conclusions:

  • Proteomic analysis provides a comprehensive view of molecular changes during liver regeneration.
  • The identified upregulated proteins represent potential targets for therapeutic interventions aimed at enhancing liver repair.
  • Partial hepatectomy triggers widespread alterations in liver proteome, affecting multiple cellular processes.