Effect of dynein inhibitor on mouse oocyte in vitro maturation and its cyclin B1 mRNA level
Related Experiment Video
Updated: Aug 20, 2026
Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
Published on: April 10, 2018
Xiao-Mei Wang1, Tian-Hua Huang, Qing-Dong Xie
1Department of Cell Biology and Genetics, Research Center for Reproductive Medicine, Shantou University Medical College, Shantou 515041, Guangdong, China. xmwang67@yahoo.com.cn
Objective:
To evaluate the effect of dynein inhibitor on mouse oocyte in vitro maturation and its cyclin B1 transcription level.
Methods:
Immature mouse oocytes were cultured in vitro with a known dynein ATPase activity inhibitor-sodium orthovanadate (SOV) to detect the changes of maturation rate, and semi-quantitative RT-PCR and single cell RT-PCR were performed to detect the changes of cyclin B1 mRNA level.
Results:
In dose-dependent experiments, the maturation rates of oocytes were significantly different between 5 micromol/L SOV and control groups (P < 0.05), and decreased with SOV increasing doses. However, the elevation of cyclin B1 mRNA level of immatured oocytes cultured for 12 h depended on SOV concentrations ranging from 50 to 500 micromol/L. In incontinuity exposed SOV experiments, the maturation rates of oocytes markedly reduced after the first incubation with 400 micromol/L SOV at least for 1 h and were first cultured in SOV-free medium for 4 h or 8 h before exposure to SOV (P < 0.05). In time-course experiment, the opposite changes of cyclin B1 mRNA level in oocytes between SOV and control groups were observed.
Conclusion:
Dynein inhibitor might delay oocytes meiosis process, and cause ectopic expression of cyclin B1 in oocytes. Most Oocytes incubated with SOV blocked at germinal vesicles (GV) stage or M I to anaphase transition due to dynein dysfunction and ectopic transcription level of cyclin B1.
