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Detection of DNA damage in individual cells by analysis of histone H2AX phosphorylation

Peggy L Olive1

  • 1Department of Medical Biophysics, British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada V5Z 1L3.

Methods in Cell Biology
|December 18, 2004
PubMed
Abstract

No abstract available in PubMed .

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In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
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